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Merck
CN

L8258

来自 多花紫藤的凝集素

lyophilized powder

别名:

多花紫藤 凝集素, WFA

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Form:
lyophilized powder
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form

lyophilized powder

Quality Level

potency

<16 μg/mL agglutination activity

composition

Protein, ~95% E1%/280

storage temp.

−20°C

General description

凝集素是一种碳水化合物结合蛋白,存在于植物的根、根茎、叶、种子和茎中。凝集素不仅存在于细胞核中,也存在于细胞质中。

Application

来自紫藤的凝集素已用于:
  • 通过免疫荧光法研究小鼠脑外神经网和小白蛋白网
  • 通过微孔板法评估N-乙酰半乳糖胺(GalNAc)转移到高斯荧光素酶(GLuc)底物的量
  • 研究对周围神经网的结合作用

Biochem/physiol Actions

WFA不是血型特异性的,但对N-乙酰基-D- 半乳糖胺具有亲和力。
凝集素与多糖、糖脂和糖蛋白的聚糖具有高亲和力。它参与植物的防御反应。

Packaging

包装尺寸基于蛋白质含量

Analysis Note

凝集活性以 μg/ml表示,并且是基于1mg/ml磷酸盐缓冲盐水(pH 6.8)的系列稀释液进行测定的。该活性是在25℃条件下温育1小时后凝集2%人血型A红细胞悬浮液的最低浓度。


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.


Nausicaä Lannoo et al.
Frontiers in plant science, 5, 397-397 (2014-08-29)
Plants are under constant attack from pathogens and herbivorous insects. To protect and defend themselves, plants evolved a multi-layered surveillance system, known as the innate immune system. Plants sense their encounters upon perception of conserved microbial structures and damage-associated patterns
Markus Morawski et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 369(1654), 20140046-20140046 (2014-09-17)
Perineuronal nets (PNs) in the brains of tenascin-R-deficient (tn-r(-/-)) mice develop in temporal concordance with those of wild-type (tn-r(+/+)) mice. However, the histological appearance of PNs is abnormal in adult tn-r(-/-) mice. Here, we investigated whether similar defects are also



全球贸易项目编号

货号GTIN
L8258-1MG04061826683682
L8258-5MG04061833272572