Quality Level
form
salt-free, lyophilized powder
potency
<10 μg per mL agglutination activity (with erythryocytes)
composition
Protein, ≥80% biuret
storage temp.
2-8°C
Biochem/physiol Actions
PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
Preparation Note
通过改良 Leavitt 方法纯化。
Analysis Note
凝集活性以 μg/ml 表示,通过 1 mg/mL 溶液的系列稀释液测定。该活性是在 25℃ 下孵育 1 h 后凝集人红细胞(2% 磷酸盐缓冲盐水中,pH 6.8)或人白细胞(10 7 /mL 盐水中)混悬液的最低浓度。
检测白细胞凝集。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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商品
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R D Leavitt et al.
The Journal of biological chemistry, 252(9), 2961-2966 (1977-05-10)
Affinity-purified phytohemagglutinin from red kidney bean resolves into five isolectins by SP-Sephadex ion exchange chromatography. Recoveries ranging from 30 to 130 mg of protein for each isolectin are easily achieved. The isolectins have similar amino acid compositions which differ only
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L8629-5MG | 04061833263709 |