form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Eagle最低必需培养基(MEM培养基)是使用最广泛的一种合成细胞培养基。MEM培养基已用于多种细胞的单层培养。通常用于在胎牛血清(FBS)、小牛或马血清存在下培养贴壁细胞，如成纤维细胞。
Application
Eagle最低必需培养基(MEM培养基)已用于：
- 原代肝细胞的分离和培养
- 培养人Ishikawa子宮內膜癌细胞(ATTC)和L-Wnt-3a细胞(ATCC)，用于分泌Wnt家族成员3a(Wnt-3a)配体
- 培养AN3CA细胞（子宫内膜癌细胞系）和HEC1-B人细胞（子宫内膜腺癌细胞系）
Features and Benefits
- Eagle最低必需培养基(MEM培养基)含有较高浓度的氨基酸，类似于哺乳动物培养细胞的蛋白组分。
- 去除钙成分后，培养基可用于悬浮细胞培养。
Preparation Note
按照每升培养基9.5克粉末配制。
补充2.2 g/L碳酸氢钠。
Other Notes
除了以粉末形式提供外，基本上与原始配方相同。
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存储类别
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M0268-25KG | 04061834408819 |
| M0268-50KG | 04061834408826 |
| M0268-10L | 04061835568772 |
| M0268-10X1L | 04061835568789 |
| M0268-1L | 04061837473128 |
| M0268-50L | 04061833992906 |