Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
它含有19种氨基酸。必需氨基酸和非必需氨基酸；L-ala、L-asn、L-asp、L-glu、L-gly、L-pro和L-ser。可能跟适合于在血清浓度降低的情况下生长贴壁细胞，或作为开发无血清培养基的基础。
最小必需培养基Eagle包含糖、无机盐、水溶性维生素和必需氨基酸。它广泛用于许多细胞培养基研究的培养基配方。它是Eagle基础培养基（BME）的改良版本，大部分氨基酸的浓度是BME的两倍。
Application
最小必需培养基Eagle已用于培养成骨样细胞系MG-63、分散心室细胞和子宫内膜腺癌Ishikawa细胞。
Preparation Note
按照每升培养基9.6克粉末配制。
补充2.2 g/L碳酸氢钠。
Still not finding the right product?
Explore all of our products under Eagle最低必需培养基
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.
Erika Nahomy Marino-Marmolejo et al.
BMC microbiology, 16(1), 290-290 (2016-12-14)
Pharmaceutical industry demands innovation for developing new molecules to improve effectiveness and safety of therapeutic medicines. Preclinical assays are the first tests performed to evaluate new therapeutic molecules using animal models. Currently, there are several models for evaluation of treatments
Hongjie Fan et al.
Cells, 10(11) (2021-11-28)
Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S
János Magyar et al.
European journal of pharmacology, 487(1-3), 29-36 (2004-03-23)
Concentration-dependent (10-1000 microM) effects of terpenoid phenol derivatives were studied on L-type Ca(2+) current in isolated canine and human ventricular cardiomyocytes using the whole-cell configuration of patch clamp technique. Carvacrol, thymol and eugenol suppressed peak Ca(2+) current at +5 mV
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M0643-50KG | 04061834408901 |
| M0643-10L | 04061833993750 |
| M0643-50K | 04061834181491 |
| M0643-25KG | 04061834408895 |
| M0643-10X1L | 04061835568802 |
| M0643-1L | 04061837459504 |
| M0643-50L | 04061833993767 |