Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes
shipped in
ambient
storage temp.
2-8°C
General description
最低必需培养基(MEM)是Harry Eagle开发的合成细胞培养基。培养基含有较高浓度的氨基酸，因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。混合Earle′s平衡盐的MEM是改良配方，又名αMEM，包含非必需氨基酸、丙酮酸钠和额外的维生素。
Application
Eagle最低必需培养基已用于：
- 自然杀伤细胞(NK)的维持培养
- 杜氏利什曼原虫(Leishmania donovani)菌株培养
- 大鼠毛囊隆突部位表皮神经嵴干细胞(EPI-NCSCs)的培养
Preparation Note
按照每升培养基10.1克粉末配制。
补充2.2 g/L碳酸氢钠。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Chia-Yen Hsiao et al.
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Lithospermi Radix (LR) is an effective traditional Chinese herb in various types of wound healing; however, its mechanism of action remains unknown. A biochemical and proteomic platform was generated to explore the biological phenomena associated with LR and its active
C C Lachaud et al.
Cell death & disease, 5, e1304-e1304 (2014-06-27)
Recent studies suggested that the post-natal mesothelium retain differentiative potential of the embryonic mesothelium, which generates fibroblasts and vascular smooth muscle cells (VSMCs), in developing coelomic organs via epithelial-to-mesenchymal transition (EMT). Whether adult mesothelial cells (MCs) are able to give
Hannah Finke et al.
Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie, 79(5), 328-336 (2018-07-18)
Miniscrews are an important choice for orthodontic anchorage. Yet reports on failures do exist, and attempts have been made to elucidate the causes. Clinical outcomes may be compromised not only by the mechanical implications of miniscrew design and the location
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M0644-10L | 04061833993781 |
| M0644-10X1L | 04061833993798 |
| M0644-BG | 04061838414595 |