grade
Molecular Biology
sterility
non-sterile; 0.2 μm filtered
form
liquid
concentration
1.00 M±0.01 M
technique(s)
DNA sequencing: suitable
impurities
DNAse, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected
solubility
water: soluble
density
1.080 g/cm3
cation traces
heavy metals (as Pb): ≤5 ppm
suitability
suitable for electrophoresis, suitable for molecular biology
application(s)
cell analysis
foreign activity
DNase, RNase, NICKase and protease, none detected
SMILES string
Cl[Mg]Cl
InChI
1S/2ClH.Mg/h2*1H;/q;;+2/p-2
InChI key
TWRXJAOTZQYOKJ-UHFFFAOYSA-L
General description
氯化镁是一种常见的离子型卤化物，易溶于水。它在酶学、细胞膜/细胞壁结构、肌肉细胞生理学和核酸结构中起多种重要的生物学作用。此外，氯化镁在许多酶中都是基本的辅酶，包括脱氧核糖核酸酶、某些限制性酶和核糖核酸酶H。氯化镁在各种分子生物学应用中（包括PCR反应）广泛用于提供镁离子。
Application
氯化镁在各种分子生物学应用，包括PCR反应和缓冲液中提供镁离子。用途：
- 配制免疫沉淀实验的细胞裂解缓冲液
- 加入Krebs-Henseleit缓冲液，用于老年小鼠的长期高脂饮食研究。
- 用于三维微生物转谷氨酰胺酶水凝胶中的细胞透化，进行骨细胞分化及互连细胞网络的体外研究。
- 保护肝脏免遭氧化损伤。
- 制备无血清培养基，用于大鼠脑切片培养物的电生理研究。
Features and Benefits
- 即用型溶液，免去配制步骤，节约时间。
- 重金属（以铅计）：≤5 ppm
- 不含DNA酶、RNA酶、切口酶和蛋白酶
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
ppe
Eyeshields, Gloves
实验方案
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。
在测定己糖激酶活性时，使用了一种在340nm处的连续分光光度法进行测定。己糖激酶使用ATP作为磷酸的来源来催化D-己糖的磷酸化。
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M1028-10X1ML | 04061834037774 |
| M1028-100ML | 04061838258427 |