产品名称
培养基 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.0-7.6 (25 °C, 209 g/L)
components
sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Application
培养基199已用于：
- 培养一岁Speckle Park母牛来源的瘤胃上皮细胞
- 作为成熟培养基组分，培养牛卵巢来源的卵丘卵母细胞复合体(COC)
- 作为杜氏改良Eagle培养基(DMEM)组分，维持新生大鼠心肌细胞(NCM)生长
Preparation Note
补充 0.1 g/L L-谷氨酰胺。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Melisa Gomez-Velazquez et al.
PLoS genetics, 13(8), e1006985-e1006985 (2017-08-29)
Cardiac progenitors are specified early in development and progressively differentiate and mature into fully functional cardiomyocytes. This process is controlled by an extensively studied transcriptional program. However, the regulatory events coordinating the progression of such program from development to maturation
Alfred Nguyen et al.
Lab on a chip, 21(10), 1929-1947 (2021-05-20)
Active microrheology is one of the main methods to determine the mechanical properties of cells and tissue, and the modelling of these viscoelastic properties is under heavy debate with many competing approaches. Most experimental methods of active microrheology such as
Martyna Pakuła et al.
International journal of molecular sciences, 20(1) (2019-01-06)
The role of the epithelial-mesenchymal transition (EMT) in ovarian cancer cell progression is unquestioned. In this report, we describe that malignant ascites, fluid that accumulates in the peritoneal cavity in a large group of patients with ovarian cancer, stimulate EMT
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M2154-6X500ML | 04061834041665 |
| M2154-500ML | 04061834041658 |