Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM
shipped in
ambient
storage temp.
2-8°C
General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Application
培养基199已用于：
- 在成熟前保持卵丘-卵母细胞复合体（COC），并培养这些卵母细胞在体外成熟
- 在德国荷斯坦奶牛卵子采集过程中清洗抽吸管
- 培养大鼠心脏内皮细胞
Preparation Note
补充 2.2 g/L 碳酸氢钠。
配制成每升培养基含 14.7 克粉末。
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Jarosław Wieczorek et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-16)
The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to
Mao Ye et al.
Scientific reports, 4, 4681-4681 (2014-04-16)
The highly specialized endothelial cells in brain capillaries are a key component of the blood-brain barrier, forming a network of tight junctions that almost completely block paracellular transport. In contrast to vascular endothelial cells in other organs, we show that
Sait Sendag et al.
Animal reproduction science, 106(1-2), 208-214 (2008-02-26)
The goal of the present study was to compare the ovarian response, oocyte yields per animal, and the morphological quality of oocytes collected by ultrasound guided follicular aspiration from Holstein cows treated either with FSH or eCG. Twenty four normal
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M2520-10X1L | 04061837847325 |