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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-743-3
NACRES:
NA.54
MDL number:
Specific activity:
1,500-3,000 units/mg protein (biuret)
Biological source:
rabbit muscle
biological source
rabbit muscle
Quality Level
form
ammonium sulfate suspension
specific activity
1,500-3,000 units/mg protein (biuret)
mol wt
21 kDa
foreign activity
3-phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase and pyruvate kinase ≤0.01%
storage temp.
2-8°C
General description
研究领域:细胞信号传导
肌激酶,也称腺苷酸激酶或ADK,是一种磷酸转移酶。它属于核苷单磷酸激酶家族。肌激酶是一种存在于骨骼肌中的酶,可以作为磷酸转移酶刺激剂。
肌激酶,也称腺苷酸激酶或ADK,是一种磷酸转移酶。它属于核苷单磷酸激酶家族。肌激酶是一种存在于骨骼肌中的酶,可以作为磷酸转移酶刺激剂。
Application
肌激酶已用于:
- 评估其与对羟基汞苯甲酸酯和对汞苯磺酸酯汞制剂的反应
- 研究其肽片段的配体结合性质
- 作为ATP生成溶液的成分用于通过AMP-荧光素酶试验定量测定3’3’-c-di-AMP
Biochem/physiol Actions
肌激酶,或称腺苷酸激酶,在细胞内能量代谢的调节以及不同细胞区室中腺嘌呤核甙酸比率的平衡中起关键作用。
肌激酶是参与腺嘌呤核甙酸代谢的关键酶。肌激酶的作用确保腺嘌呤核苷酸(ATP、ADP和AMP)在胞质溶胶中保持平衡。
肌激酶催化以下反应:ADP + ADP → ATP + AMP
肌激酶是参与腺嘌呤核甙酸代谢的关键酶。肌激酶的作用确保腺嘌呤核苷酸(ATP、ADP和AMP)在胞质溶胶中保持平衡。
肌激酶催化以下反应:ADP + ADP → ATP + AMP
使用二硫双(2-硝基苯甲酸)处理,可以使兔肌肉肌激酶失活,因为它能改变对肌激酶活性至关重要的蛋氨酸残基。
Physical form
悬浮在3.2M(NH4)2SO4溶液中,pH 6.0,含有0.001 M EDTA
Other Notes
一个酶活性单位是指在pH 7.6、37°C下,每分钟将2.0 μmole的ADP转化为ATP + AMP所需的酶量。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Inactivation of rabbit muscle adenosine triphosphate-adenosine 5'-phosphate phosphotransferase by alkylation of methionine residues.
L F Kress et al.
The Journal of biological chemistry, 242(4), 558-564 (1967-02-25)
Likui Feng et al.
Nature communications, 12(1), 4912-4912 (2021-08-15)
Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) hybrid systems typically use complex protein-protein interactions to facilitate direct transfer of intermediates between these multimodular megaenzymes. In the canal-associated neurons (CANs) of Caenorhabditis elegans, PKS-1 and NRPS-1 produce the nemamides, the only known
Qingfeng Chen et al.
The Journal of biological chemistry, 285(39), 29780-29786 (2010-07-20)
The GH3 family of acyl-acid-amido synthetases catalyze the ATP-dependent formation of amino acid conjugates to modulate levels of active plant hormones, including auxins and jasmonates. Initial biochemical studies of various GH3s show that these enzymes group into three families based
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M3003-10KU | 04061832924519 |
| M3003-5KU | 04061832924540 |
| M3003-1KU | 04061832924526 |
| M3003-2.5KU | 04061834046295 |