Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no
shipped in
ambient
storage temp.
2-8°C
General description
Eagle最低必需培养基含有19种氨基酸。包括必需氨基酸和非必需氨基酸：丙氨酸(L-ala)、天冬酰胺(L-asn)、天冬氨酸(L-asp)、谷氨酸(L-glu)、甘氨酸(L-gly)、脯氨酸(L-pro)和丝氨酸(L-ser)。可优先用于在减少的血清存在下的贴壁细胞的生长或作为开发无血清培养基的基础配方。适合多种细胞的单层培养。
Application
Eagle最低必需培养基已用于：
- 培养Ishikawa子宮內膜腺癌细胞，用于双酚研究
- 培养MCF-7细胞，用于激素处理实验
- 维持斑马鱼松果体活性，用于作用光谱分析
Features and Benefits
含有高浓度的氨基酸，因此培养基成分类似于哺乳动物培养细胞的蛋白质成分。
Preparation Note
补充 0.292 g/L左旋谷氨酰胺、2.2 g/L 碳酸氢钠。
配制成每升培养基含 9.3 克粉末。
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存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Anatoli Y Kabakov et al.
PloS one, 10(4), e0123718-e0123718 (2015-04-24)
During long-term potentiation (LTP) of excitatory synapses, Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is activated by Ca(2+) influx through NMDA receptors that potentiate AMPA receptor currents by insertion of additional GluR1-containing receptors at the synapse and by increasing AMPA channel conductance
Kyle S Enger et al.
Journal of water and health, 14(6), 950-960 (2016-12-14)
Many different household water treatment (HWT) methods have been researched and promoted to mitigate the serious burden of diarrheal disease in developing countries. However, HWT methods using bromine have not been extensively evaluated. Two gravity-fed HWT devices (AquaSure™ and Waterbird™)
Hongjie Fan et al.
Cells, 10(11) (2021-11-28)
Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M3024-10L | 04061834046301 |
| M3024-10X1L | 04061834046318 |
| M3024-1L | 04061837473449 |