Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Application
培养基199已用于：
- 培养原代人脐静脉内皮细胞（HUVEC），以用于细胞活力检测
- 冲洗小母猪子宫角，从子宫恢复第12天妊娠
- 培养腹膜细胞和永生化人间皮细胞（MeT-5A和ATCC-CRL-9444）
Preparation Note
补充0.1克/升左旋谷氨酰胺、2.2克/升碳酸氢钠。
配制成每升培养基中含 9.4 克粉末。
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存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Agnieszka Blitek et al.
Theriogenology, 127, 88-101 (2019-01-25)
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor family of ligand-dependent transcription factors. PPARs are important regulators of glucose and fatty acid metabolism, apoptosis, angiogenesis, cell proliferation and differentiation, and immune response. Their possible role in the female
Vania Cristina Santos et al.
PloS one, 9(11), e111241-e111241 (2014-11-05)
Some reports have described the interference of Leishmania on sand flies physiology, and such behavior most likely evolved to favor the development and transmission of the parasite. Most of these studies showed that Leishmania could modulate the level of proteases
Diane G Edmondson et al.
mBio, 12(1) (2021-02-25)
The bacterium that causes syphilis, Treponema pallidum subsp. pallidum, has now been cultured in vitro continuously for periods exceeding 3 years using a system consisting of coculture with Sf1Ep rabbit epithelial cells in TpCM-2 medium and a low-oxygen environment. In
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M3769-50L | 04061834050568 |
| M3769-10X1L | 04061834050551 |