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Merck
CN

M4526

Eagle最低必需培养基

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

别名:

αMEM, MEM

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.71
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产品名称

Eagle最低必需培养基, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

shipped in

ambient

storage temp.

2-8°C

General description

最小必需培养基Eagle(MEM)是一种合成细胞培养基,由Harry Eagle开发。它有更高浓度的氨基酸,所以培养基更接近培养哺乳动物细胞的蛋白质组成。在含有Hanks或Earle盐的配方中选择补充非必需氨基酸,可增强该培养基的用途。Earle平衡盐的MEM修饰也称为αMEM,含有非必需氨基酸、丙酮酸钠和其他维生素。
这是所提供的MEM配方中最丰富的变体。它含有所有21种正常氨基酸,其中一些的浓度有所增加。此外,它还含有5种额外的维生素。

Application

最小必需培养基Eagle已用于维持:
  • 小鼠未成熟的卵母细胞络合物
  • 人类间充质干细胞(MSC)
  • 小鼠成骨前细胞系

Preparation Note

添加 0.292g/L L-谷氨酰胺。


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

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全球贸易项目编号

货号GTIN
M4526-500ML04061834059004
M4526-24X500ML04061834058991
M4526-6X500ML04061834059011