产品名称
Eagle最低必需培养基, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no
shipped in
ambient
storage temp.
2-8°C
General description
Eagle最低必需培养基(MEM)是Harry Eagle开发的人工合成细胞培养基。培养基含有较高浓度的氨基酸，因此比较接近哺乳动物培养细胞的蛋白质浓度。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。去除钙成分后，可用于悬浮细胞培养。它是经典MEM配方的改良版。厄尔′盐类已被汉克′盐类所取代。由于碳酸氢盐浓度显著降低，因此该介质具有显著降低的缓冲能力。
Application
Eagle最低必需培养基已用于：
- 作为Hank′s MEM(HMEM)-PIPES缓冲液成分，用于小鼠中过敏毒素(anaphylatoxin)诱导耳肿的引发
- 作为小鼠海马体切片培养基(SCM)组分
- 培养豚鼠Müller细胞
Preparation Note
按照每升培养基10.6克粉末配制。
补充0.35 g/L碳酸氢钠。
Still not finding the right product?
Explore all of our products under Eagle最低必需培养基
存储类别
11 - Combustible Solids
wgk
WGK 1
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Anthony Dudilot et al.
Current biology : CB, 30(7), 1167-1176 (2020-02-23)
Synaptic plasticity, with its two most studied forms, long-term potentiation (LTP) and long-term depression (LTD), is the cellular mechanism underlying learning and memory. Although it has been known for two decades that bidirectional synaptic plasticity necessitates a corresponding bidirectional regulation
Pasqualino de Antonellis et al.
PloS one, 6(9), e24584-e24584 (2011-09-21)
Through negative regulation of gene expression, microRNAs (miRNAs) can function as oncosuppressors in cancers, and can themselves show altered expression in various tumor types. Here, we have investigated medulloblastoma tumors (MBs), which arise from an early impairment of developmental processes
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M4642-50L | 04061834059103 |
| M4642-25KG | 04061834409069 |
| M4642-50KG | 04061834409076 |
| M4642-10L | 04061834059097 |
| M4642-50K | 04061834171935 |