Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
由Harry Eagle开发的最低必需培养基（MEM）是所有合成细胞培养基中使用最广泛的培养基之一。早期在尝试培养正常的哺乳动物成纤维细胞和HeLa细胞的某些亚型的过程中，显示它们具有Eagle's基础培养基（BME）无法满足的特定营养需求。随后在培养中使用这些细胞和其他细胞的研究表明，通过向BME中加入添加物以帮助更广泛的苛刻细胞的生长。
通过改良的MEM包括更高浓度的氨基酸，因此该培养基更接近于所培养的哺乳动物细胞的蛋白质组成。MEM已用于在单层中生长的各种细胞的培养。在含有Hanks'或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。
通过改良的MEM包括更高浓度的氨基酸，因此该培养基更接近于所培养的哺乳动物细胞的蛋白质组成。MEM已用于在单层中生长的各种细胞的培养。在含有Hanks'或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。
Application
通常用于在FBS、小牛或马血清存在下生长附着的细胞系，例如成纤维细胞。
Other Notes
该产品中缺乏L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Yongjun Chu et al.
Nucleic acids research, 48(12), 6740-6758 (2020-05-29)
How genetic defects trigger the molecular changes that cause late-onset disease is important for understanding disease progression and therapeutic development. Fuchs' endothelial corneal dystrophy (FECD) is an RNA-mediated disease caused by a trinucleotide CTG expansion in an intron within the
Jianguo Huang et al.
Nature communications, 8, 15999-15999 (2017-07-12)
Genetically engineered mouse models that employ site-specific recombinase technology are important tools for cancer research but can be costly and time-consuming. The CRISPR-Cas9 system has been adapted to generate autochthonous tumours in mice, but how these tumours compare to tumours
Jiaxin Hu et al.
Biochemistry, 53(28), 4510-4518 (2014-07-02)
Dentatorubral-pallidoluysian atrophy (DRPLA) is a progressive neurodegenerative disorder that currently has no curative treatments. DRPLA is caused by an expansion of a CAG trinucleotide repeat region within the protein-encoding sequence of the atrophin-1 (ATN-1) gene. Inhibition of mutant ATN-1 protein
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M4655-500ML | 04061837847387 |
| M4655-6X1L | 04061834059134 |
| M4655-10L | 04061837340796 |
| M4655-1L | 04061834059110 |
| M4655-24X500ML | 04061834059127 |
| M4655-6X500ML | 04061837847394 |