产品名称
Eagle最低必需培养基, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
General description
Eagle最低必需培养基(MEM)是Harry Eagle开发的人工合成细胞培养基。培养基含有较高浓度的氨基酸，因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。通过选择性去除钙成分后，该配方可进一步改良为适合悬浮细胞培养。MEM可用于各种细胞的单层培养。
Application
Eagle最低必需培养基已用于培养：
此培养基在血清减量条件下适合贴壁细胞生长，也可作为无血清培养基开发的基础配方。
- HeLa细胞
- Madin-Darby犬肾细胞 (MDCK)
- 软骨肉瘤细胞系
此培养基在血清减量条件下适合贴壁细胞生长，也可作为无血清培养基开发的基础配方。
Preparation Note
添加 0.292g/L L-谷氨酰胺。
Still not finding the right product?
Explore all of our products under Eagle最低必需培养基
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Christopher G Mills et al.
Scientific reports, 7(1), 14824-14824 (2017-11-03)
We present a strategy for increasing the anatomical realism of organoids by applying asymmetric cues to mimic spatial information that is present in natural embryonic development, and demonstrate it using mouse kidney organoids. Existing methods for making kidney organoids in
C-Hong Chang et al.
Journal of anatomy, 235(2), 262-270 (2019-05-18)
The path taken by the loop of Henle, from renal cortex to medulla and back, is critical to the ability of the kidney to concentrate urine and recover water. Unlike most developing tubules, which navigate as blind-ended cylinders, the loop
Kaja Bergant Loboda et al.
Journal of chemical information and modeling, 60(7), 3662-3678 (2020-06-03)
Human type II topoisomerases, molecular motors that alter the DNA topology, are a major target of modern chemotherapy. Groups of catalytic inhibitors represent a new approach to overcome the known limitations of topoisomerase II poisons such as cardiotoxicity and induction
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M5650-6X500ML | 04061834060178 |
| M5650-1L | 04061834060154 |
| M5650-500ML | 04061834060161 |