Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | embryo: suitable
impurities
endotoxin, tested
components
NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
Application
M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的 Krebs-Ringer 碳酸氢盐溶液，与 Whitten′s 培养基非常相似。M16 含有丙酮酸和乳酸作为能量来源，因为植入前胚胎不能有效利用葡萄糖。
Preparation Note
添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。
Analysis Note
溶液中的沉淀不影响本品性能。
该产品经过测试，能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80％。
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存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动物来源培养基
此项目有
商品
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
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Oncotarget, 7(2), 1155-1167 (2015-12-31)
Mitochondrial dynamics change mitochondrial morphological features and numbers as a part of adaptive cellular metabolism, which is vital for most eukaryotic cells and organisms. A disease or even death of an animal can occur if these dynamics are disrupted. Using
Marie-Christine Birling et al.
Scientific reports, 7, 43331-43331 (2017-03-08)
Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of
Qian-Qian Sha et al.
Nature communications, 9(1), 3477-3477 (2018-08-30)
Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M7292-100ML | 04061834061854 |
| M7292-50ML | 04061834061878 |