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Merck
CN

M8403

McCoy 5A培养基

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

5A basal medium

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
EC Number:
200-655-4
MDL number:
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产品名称

McCoy 5A培养基, Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

shipped in

ambient

storage temp.

2-8°C

General description

McCoy 5A培养基适用于各种组织细胞原代培养。推荐用于在原代细胞培养中生产病毒。

Application

McCoy 5A培养基已用于维持:

  • G-361黑色素瘤细胞;
  • 87-MG细胞
  • 人结肠癌细胞系HCT116

Biochem/physiol Actions

McCoys 5A培养基起初是通过修改BME中的氨基酸,用来支持肝肿瘤细胞。 该配方还被用来支持骨髓、皮肤、牙龈、肾脏、大网膜、肾上腺、肺、脾、大鼠胚胎和其他细胞类型原代培养的生长。

Preparation Note

补充 0.22 g/L L-谷氨酰胺。


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动物来源培养基

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

McCoy's 5A Modified Media Formulation


A-P Sappino et al.
Oncogenesis, 1, e7-e7 (2012-01-01)
The p53 tumor-suppressor protein has a key role in the induction of cellular senescence, an important barrier to cancer development. However, very little is known about the physiological mediators of cellular senescence induced by p53. CEACAM1 is an immunoglobulin superfamily
Roman A Perez et al.
Journal of tissue engineering, 5, 2041731414543965-2041731414543965 (2014-11-11)
Developing appropriate cell culturing techniques to populate scaffolds has become a great challenge in tissue engineering. This work describes the use of spinner flask dynamic cell cultures to populate hydroxyapatite microcarriers for bone tissue engineering. The microcarriers were obtained through
Huw D Summers et al.
ACS nano, 7(7), 6129-6137 (2013-06-19)
Assessing dose in nanoparticle-cell interactions is inherently difficult due to a complex multiplicity of possible mechanisms and metrics controlling particle uptake. The fundamental unit of nanoparticle dose is the number of particles internalized per cell; we show that this can



全球贸易项目编号

货号GTIN
M8403-24X500ML04061834063049
M8403-500ML04061837847417
M8403-6X500ML04061834063056