M9067
Anti-MRP1 antibody,Mouse monoclonal
clone QCRL-1, purified from hybridoma cell culture
别名:
Anti-Multidrug Resistance Associated Protein 1
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关于此项目
Conjugate:
unconjugated
Clone:
QCRL-1, monoclonal
Application:
FACS, ICC, IHC, IP, WB
Citations:
6
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
QCRL-1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~190 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
flow cytometry: suitable, immunocytochemistry: 10-20 μg/mL using cultured HeLa cells or HepG2 human hepatocellular carcinoma cells, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ABCC1(4363)
General description
Monoclonal Anti-MRP1 (mouse IgG1 isotype) is derived from the QCRL-1 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from mice immunized with non-denatured membrane preparations of human H69AR small cell lung cancer cell line, which highly expresses MRP1.
Multidrug resistance associated protein 1 (MRP1) is present in subcellular vesicles and is composed of 1531 amino acids. It has a molecular weight of 190kDa. The gene encoding MRP1 is localized in human chromosome 16p13.11.
Immunogen
non-denatured membrane preparations of H69AR human small cell lung cancer cell line, which highly expresses MRP1. The epitope resides within amino acids 918-924 of human MRP1.
Application
Monoclonal Anti-MRP1 antibody produced in mouse has been used in:
- western blotting
- immunohistochemistry
- flow cytometry
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
Does not cross-react with the human MDR1 and MDR3, mouse MRP1, and human MRP2, MRP3, MRP4, MRP5, and MRP6 gene products. The antibody may cross-react with canine MRP1.
Multidrug resistance associated protein 1 (MRP1) functions in the metabolism of many drugs. It is involved in transporting different biomolecules and anticancer drugs like prostaglandins, glutathione, alkaloids and anthracyclines. MRP1 offers resistance to chemotherapy to cells in solid tumors.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Characterization of three B-cell lymphoma cell lines from chemotherapy resistant patients with respect to in vitro sensitivity to 21 antitumor agents, ABC-transporter expression and cellular redox status
Bracht K, et al.
Journal of Cancer Research and Clinical Oncology, 133(12), 957-967 (2007)
Shu-Li Shao et al.
Asian Pacific journal of cancer prevention : APJCP, 15(24), 10597-10601 (2015-01-22)
Up-regulation of multidrug resistance-associated protein 1 (MRP1) is regarded as one of the main causes for multidrug resistance (MDR) of tumor cells, leading to failure of chemotherapy-based treatment for a multitude of cancers. However, whether silencing the overexpressed MRP1 is
Aaron Black et al.
The journal of gene medicine, 16(5-6), 122-130 (2014-06-26)
Choroideremia (CHM) is a slowly progressive X-linked retinal degeneration that results in a loss of photoreceptors, retinal pigment epithelium and choroid. CHM, the gene implicated in choroideremia, encodes Rab escort protein-1 (REP-1), which is involved in the post-translational activation via
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M9067-200UL | 04061838106315 |