M9901
变溶菌素 来源于球孢链霉菌 ATCC 21553
lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
别名:
Mutanolysin
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关于此项目
Specific activity:
≥4000 units/mg protein (biuret)
Biological source:
bacterial (Streptomyces globisporus)
biological source
bacterial (Streptomyces globisporus)
Quality Level
form
lyophilized powder
specific activity
≥4000 units/mg protein (biuret)
mol wt
23 kDa
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
从 链霉菌 中提取的变溶菌素由两种主要的裂解酶组成，可能是一种有效的龋病防治剂。
Application
诱变溶菌素在一项研究中被用于评估牛奶 链球菌的裂解和原生质体的生成。 它也在一项研究中被用于研究研究B类 链球菌 向原生质体的转化。 它为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
Biochem/physiol Actions
变溶菌素可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。
变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶，可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。
Physical form
冻干粉，含Ficoll® 和琥珀酸钠缓冲盐
Other Notes
以粪链球菌细胞壁混悬液为底物，在1mL体积中，1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600 。
Legal Information
Ficoll is a registered trademark of Cytiva
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
13 - Non Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
实验方案
的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。
在测定变溶菌素活性时，使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M9901-1KU | 04061835557806 |
| M9901-10KU | 04061835557790 |
| M9901-50KU | 04061835557813 |
| M9901-5KU | 04061835557820 |