usage
sufficient for 200 fluorometric tests (orange)
application(s)
cosmetics
food and beverages
detection method
fluorometric
relevant disease(s)
cancer; neurological disorders
storage temp.
−20°C
General description
在有氧呼吸期间氧气减少以及在细胞内各种酶体系的作用下,可生成活性氧类物质(ROS)。在生理水平上,ROS有助于细胞信号传导和宿主防御能力。当ROS生成增加至超过生物系统的解毒能力时,将导致氧化应激和细胞损伤。对细胞的主要损伤来源于ROS诱导的大分子改变,如膜脂质的多不饱和脂肪酸、必需蛋白质和DNA。已有研究证明ROS与疾病状态有关,如阿尔茨海默病、帕金森病、癌症和衰老。
Application
细胞内活性氧类物质(ROS)检测试剂盒(荧光法)已可用于评估机械通气(MV)对线粒体的损害。它还被用于测量细胞内ROS的产生。
本试剂盒适于通过酶标仪、流式细胞仪或带TRITC滤镜的荧光显微镜检测细胞内的ROS。
Biochem/physiol Actions
细胞内ROS检测试剂盒(荧光法)是一种灵敏的一步式荧光测定法,仅需1小时孵育即可检测活细胞中的细胞内ROS(特别是超氧化物和羟基自由基)。ROS与细胞可透过性传感器反应,产生的荧光产物(λex = 540/λem = 570 nm)与ROS含量成比例。该测定可使用方便的96孔或384孔微量滴定板。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Oxidized low density lipoprotein (ox-LDL) binding to ox-LDL receptor-1 in endothelial cells induces the activation of NF-KB through an increased production of intracellular reactive oxygen species.
Cominacini L, et al.
The Journal of Biological Chemistry, 275(17), 12633-12638 (2000)
High tidal volume induces mitochondria damage and releases mitochondrial DNA to aggravate the ventilator-induced lung injury.
Lin J, et al.
Frontiers in Immunology, 9, 1477-1477 (2018)
Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants.
Das K and Roychoudhury A
Frontiers in environmental science., 2, 53-53 (2014)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAK144-1KT | 04061834376323 |