Acid Phosphatase Assay Kit

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

The Acid Phosphatase Assay Kit may be used for:

• Cancer Research
• Gastrointestinal Research
• Metabolism Research

The kit is suitable for the determination of acid phosphatase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)

The non-radioactive, colorimetric Acid Phosphatase Assay Kit is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

Efficient and Precise: Requires only 20 μL of sample with a 30-minute assay reaction to detect acid phosphatase levels across a broad range from 0.05 to 60 U/L, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

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