N2640
4-硝基苯基磷酸盐 二钠盐 六水合物
chromogenic, tablet
别名:
4-硝基苯磷酸二钠 六水合物, pNPP 二钠 六水合物, 对硝基苯磷酸二钠, 对硝基苯磷酸盐 二钠 六水合物
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
线性分子式:
O2NC6H4OP(O)(ONa)2 · 6H2O
化学文摘社编号:
分子量:
371.14
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
Beilstein/REAXYS Number:
5324661
产品名称
4-硝基苯基磷酸盐 二钠盐 六水合物, tablet
form
tablet
Quality Level
solubility
water: 1 tablet/3 mL, clear, colorless
storage temp.
−20°C
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
General description
用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应，并在 405nm 下读数。
Application
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
Preparation Note
Contains 15 mg substrate per tablet
Still not finding the right product?
Explore all of our products under 4-硝基苯基磷酸盐 二钠盐 六水合物
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Vitamin A differentially regulates cytokine expression in respiratory epithelial and macrophage cell lines
Penkert RR, et al.
Cytokine, 91, 1-5 (2017)
Alkaline Phosphatase Assay Based on the Chromogenic Interaction of Diethanolamine with 4-Aminophenol
Sun J, et al.
Analytical Chemistry, 90(10), 6339-6345 (2018)
Protein tyrosine phosphatase assays
Lorenz U
Current Protocols in Immunology, 93(1), 11-17 (2011)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N2640-50TAB | 04061835557974 |
| N2640-100TAB | 04061835557967 |