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Merck
CN

N2765

4-硝基苯基磷酸盐 二钠盐 六水合物

chromogenic, tablet

别名:

4-硝基苯磷酸二钠 六水合物, pNPP 二钠 六水合物, 对硝基苯磷酸二钠, 对硝基苯磷酸盐 二钠 六水合物

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关于此项目

线性分子式:
O2NC6H4OP(O)(ONa)2 · 6H2O
化学文摘社编号:
分子量:
371.14
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
Beilstein/REAXYS Number:
5324661
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产品名称

4-硝基苯基磷酸盐 二钠盐 六水合物, tablet

form

tablet

Quality Level

solubility

water, high purity: 0.25 tablet/mL, clear, colorless

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

General description

用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应,并在 405nm 下读数。

Application

4-硝基苯磷酸二钠盐六水合物已用于:
  • 在酶联免疫吸附测定(ELISA)中作为碱性磷酸酶偶联抗兔抗体的底物
  • 在酶标免疫实验中作为碱性磷酸酶标记小鼠二抗的底物
  • ELISA法分析动物血清样本中铜绿假单胞菌抗体的存在

Preparation Note

每片含有20mg底物


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.


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全球贸易项目编号

货号GTIN
N2765-100TAB04061835546237
N2765-5X100TAB04061834110484
N2765-50TAB04061834110477