产品名称
SIGMAFAST™ 对硝基苯基磷酸酯片, tablet, To prepare 20 mL
Quality Level
form
tablet
solubility
water: 0.25 tablet/mL, clear, colorless
storage temp.
−20°C
Application
开发用作可溶性底物，用于检测酶免疫分析法（EIA）中碱性磷酸酶的活性。 pNPP是高灵敏度碱性磷酸酶EIA检测的首选底物。将一片pNPP和一片Tris缓冲片溶解在去离子水中即得即用型底物。 SIGMAFAST pNPP每片可产生含有1.0 mg/ml pNPP、0.2 M Tris缓冲液的溶液。使用pNPP的EIA应用可以在定时实验中读数，或用3 N NaOH终止反应后延迟读数。SIGMAFAST™ 仅需要添加蒸馏水或去离子水即可制备活性底物溶液。片剂用箔包单个包装。
Preparation Note
将每片片剂溶于 20 mL 去离子水中，得到含 pNPP 的即用型缓冲液。
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N2770-5SET | 04061834110491 |
| N2770-50SET | 04061835557998 |