N7653
对硝基苯磷酸盐液体底物系统
alkaline phosphatase substrate, liquid
别名:
4-硝基苯磷酸二钠 溶液, pNPP
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关于此项目
线性分子式:
O2NC6H4OP(O)(ONa)2
化学文摘社编号:
分子量:
263.05
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
产品名称
对硝基苯磷酸盐液体底物系统, liquid
form
liquid
Quality Level
shipped in
wet ice
storage temp.
−20°C
SMILES string
[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
InChI key
VIYFPAMJCJLZKD-UHFFFAOYSA-L
General description
对硝基苯磷酸盐（pNPP）液体底物系统将对硝基苯磷酸盐、缓冲液和所需的镁阳离子结合在一起，成为一种便捷的即用型单一溶液试剂。pNPP能够产生可溶性终产物，是碱性磷酸酶免疫测定的理想底物。
Application
在碱性磷酸酶测定中，采用p-对硝基苯基磷酸盐液体底物体系作为测定碱性磷酸酶活性的底物。
碱性磷酸酶的底物，能够产生可在405 nm处读取的可溶性黄色终产物。使用3N NaOH可终止反应并在405 nm处读取结果。推荐用于ELISA（微孔）分析，不建议用于膜应用。
Biochem/physiol Actions
4-硝基苯基磷酸二钠盐六水(pNPP)是测定蛋白质酪氨酸磷酸酶活性的非蛋白质和非特异性底物。pNPP水解后会生成p-硝基酚。它是碱性磷酸酶常见的显色底物。
Physical form
即用型。
Disclaimer
可在低于0°C的环境中稳定储存至少一年。
signalword
Danger
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
target_organs
Kidney,Liver,Blood
存储类别
12 - Non Combustible Liquids
商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N7653-100ML | 04061838256126 |