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N8016

2-Nitrophenyl β-D-glucopyranoside

Name: 2-Nitrophenyl &#946;-<SC>D</SC>-glucopyranoside
Brand: SIGMA

≥99% (TLC), powder

别名:

2-Nitrophenyl-beta-D-glucopyranoside, o-NPG

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关于此项目

经验公式（希尔记法）:

C₁₂H₁₅NO₈

化学文摘社编号:

2816-24-2

分子量:

301.25

NACRES:

NA.28

PubChem Substance ID:

24897848

UNSPSC Code:

12352204

EC Number:

220-568-5

MDL number:

MFCD00065048

Beilstein/REAXYS Number:

92206

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属性

产品名称

2-Nitrophenyl β-D-glucopyranoside, powder

assay

≥99% (TLC)

Quality Level

300

form

powder

solubility

water: 50 mg/mL

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11-,12-/m1/s1

InChI key

KUWPCJHYPSUOFW-RMPHRYRLSA-N

说明

Application

2-Nitrophenyl β-D-glucopyranoside has been used as a substrate for glucosidase in protein assay and in a colorimetric assay to determine protein stability.

Biochem/physiol Actions

2-Nitrophenyl β-D-glucopyranoside serves as an artificial substrate for glucosidase.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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商品

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Detection of inactive precursors of beta-glucanases in Saccharomyces cerevisiae.

L M Hernandez et al.

FEBS letters, 161(2), 190-194 (1983-09-19)

Accumulation and secretion of beta-glucanases have been studied in vivo by using a thermosensitive secretory mutant of Saccharomyces cerevisiae blocked at the endoplasmic reticulum level (sec 18-1). When incubated at the restrictive temperature no accumulation of active glucanases was observed.

beta-Glucosidase as a reporter for the gene expression studies in Thermus thermophilus and constitutive expression of DNA repair genes.

Toshihiro Ohta et al.

Mutagenesis, 21(4), 255-260 (2006-06-17)

Thermus thermophilus is an extremely thermophilic eubacterium that grows optimally at 70-75 degrees C. Because the frequency of DNA damage, such as deamination, depurination and single-strand breaks, increases as the temperature rises, the regulation of expression as well as the

Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles.

M Moracci et al.

Biochemistry, 37(49), 17262-17270 (1998-12-23)

The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-glycosides following a retaining mechanism based upon the action of two amino acids: Glu387, which acts as the nucleophile of the reaction, and Glu206, which acts as the general acid/base catalyst.

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全球贸易项目编号

货号	GTIN
N8016-250MG	04061834118350
N8016-1G	04061826692158