usage
sufficient for 4 purifications
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
test parameters
sample volume: 150 mL bacterial culture
greener alternative category
storage temp.
15-25°C
General description
GenElute HP质粒大量制备试剂盒可以简单、快速、低成本的从重组大肠杆菌培养物中分离质粒DNA。该试剂盒采用了一种可快速清除裂解液的过滤针筒和一种适于真空或离心形式的硅胶结合柱。从150 mL Luria Broth (LB)培养基过夜培养物中，可分离出多达1.2 mg 的质粒DNA。请注意，实际得率取决于所用的菌株、质粒和培养基。
通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。通过两遍洗涤步骤，去除污染物。最后，在洗脱溶液(Tris-HCl)或水中洗脱结合所得的DNA。
回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接供限制性酶切、连接、测序、PCR、转化和转染等下游应用使用。
通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。通过两遍洗涤步骤，去除污染物。最后，在洗脱溶液(Tris-HCl)或水中洗脱结合所得的DNA。
回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接供限制性酶切、连接、测序、PCR、转化和转染等下游应用使用。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法，本产品符合固有安全化学原则(Inherently Safer Chemistry)。
Application
GenElute™ HP Plasmid Maxiprep Kit has been used to isolate plasmid DNA from Escherichia coli.
Features and Benefits
- 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
- 可纯化出多达1.2 mg的高拷贝数质粒DNA
- 真空或离心形式可灵活选择
- 相对于其他快速试剂盒，所含的塑料成分更少，减少了废物量
- 无需进行苯酚/氯仿萃取或乙醇沉淀步骤
Other Notes
更多信息，请见www.sigma-aldrich.com/genelutehp。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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signalword
Danger
target_organs
Central nervous system
存储类别
3 - Flammable liquids
flash_point_f
77.0 °F - closed cup
flash_point_c
25 °C - closed cup
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
法规信息
危险化学品
低风险生物材料
此项目有
实验方案
Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.
Development of novel plasmid vectors and a promoter trap system in Francisella tularensis compatible with the pFLN10 based plasmids
David A Rasko
Plasmid, 58(2) (2007)
Ilaria Mogno et al.
Genome research, 23(11), 1908-1915 (2013-08-08)
Gene promoters typically contain multiple transcription factor binding sites (TFBSs), which may vary in affinity for their cognate transcription factors (TFs). One major challenge in studying cis-regulation is to understand how TFBS variants affect gene expression. We studied the in
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| NA0300S-1KT | 04061834119029 |