General description
天然蛋白去糖基化设计用于 PNGase F 抗性天然蛋白 N-连接寡糖的去糖基化。内切糖苷酶 F1、F2 和 F3 对蛋白质构象的敏感性低于 PNGase F，更适用于去除所有类别的 N-连接寡糖而不使蛋白质变性。
用神经氨酸酶、 β-半乳糖苷酶和 N-乙酰氨基葡萄糖苷酶处理糖蛋白，可将所有复杂的寡糖还原为三甘露糖基壳二糖核心。在某些情况下可能需要岩藻糖苷酶。残留的三甘露糖基壳二糖核心结构则可通过内切糖苷酶F3去除。内切糖苷酶F2和F3可即刻分别去除双触角和三触角结构。内切糖苷酶F1可去除低聚甘露糖和杂合结构。
每种类型内切糖苷酶的更多相关信息，请参阅公告。
每种类型内切糖苷酶的更多相关信息，请参阅公告。
Application
天然蛋白去糖基化试剂盒用于各种酶的 N-去糖基化，适用漆酶、 番茄核酸酶TBN1和内切-β-1,3-葡聚糖酶。
Preparation Note
NDEGLY 套件用湿冰运输，建议储存在2°C至8°C条件下。如按照指示说明储存，则该套件有效期至少为一年。只在环境温度下暴露几天，不会降低该试剂盒中酶的活性。
仅试剂盒组分
产品编号
说明
- Endoglycosidase F1 .3 U
- Endoglycosidase F2 .1 U
- Endoglycosidase F3 .1 U
- Endoglycosidase F1 reaction buffer 200 μL
- Endoglycosidase F2 & 3 reaction buffer 200 μL
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
Protein modifications are crucial for disease study. Analysis methods are key.
相关内容
Chaouki Benabdessalem et al.
Biochemical and biophysical research communications, 516(3), 845-850 (2019-07-03)
We previously reported that immunoreactivity of recombinant CFP32 (Rv0577), a virulence factor of Mycobacterium tuberculosis, was higher when produced in transformed Pichia pastoris as compared to transformed E. coli. In this study, we show that this difference is partly due to
M P Kolomytseva et al.
International journal of biological macromolecules, 137, 1199-1210 (2019-07-12)
Three laccase isoforms with different physicochemical properties could be purified from culture liquid of basidiomycete Lentinus strigosus 1566 obtained during submerged cultivation. The purified laccases possessed individual selectivity in relation to different phenolic compounds. Laccases I, II, and III (59
N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity
Podzimek T, et al.
Plant Science (2018)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| NDEGLY-1KT | 04061834119128 |