sterility
aseptically filled
Quality Level
form
colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )
technique(s)
cell culture | mammalian: suitable
color
clear colorless to faint yellow
pH
8.5-9.5 (20 °C)
application(s)
hematology
histology
storage temp.
2-8°C
General description
Percoll®是一种用于细胞、病毒和亚细胞颗粒密度梯度离心的经典培养基。Percoll由直径为15-30 nm（在水中为23％ w/w）并涂覆有聚乙烯吡咯烷酮（PVP）的胶体二氧化硅颗粒组成。PVP涂层使产品完全无毒，非常适合与生物材料一起使用。
Application
Percoll®已用于在精子准备中以研究牛胚胎的能量需求。
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成，这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用于细胞分离和细胞器分离。
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成，这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用来建立无毒低粘度，低渗透压密度梯度，可用于分离细胞，细胞器和病毒。它已被用来分离 大肠杆菌的内外膜泡。 Percoll在整个梯度过程中都是等渗的，无毒，并且很容易从纯化的材料中去除。
Legal Information
Percoll is a registered trademark of Cytiva
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
实验方案
为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
商品
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
T Hirano et al.
The Journal of cell biology, 106(4), 1171-1183 (1988-04-01)
A temperature-sensitive mutant nuc2-663 of the fission yeast Schizosaccharomyces pombe specifically blocks mitotic spindle elongation at restrictive temperature so that nuclei in arrested cells contain a short uniform spindle (approximately 3-micron long), which runs through a metaphase plate-like structure consisting
Rajasree Sreedharan et al.
Kidney international, 86(3), 515-524 (2014-05-09)
Inducible heat shock proteins (HSPs), regulated by heat shock factor-1 (HSF-1), protect against renal cell injury in vitro. To determine whether HSPs ameliorate ischemic renal injury in vivo, HSF-1 functional knockout mice (HSF-KO) were compared with wild-type mice following bilateral
Guilherme D Melo et al.
Scientific reports, 7(1), 8454-8454 (2017-08-18)
Visceral leishmaniasis (VL) is a systemic disease with multifaceted clinical manifestations, including neurological signs, however, the involvement of the nervous system during VL is underestimated. Accordingly, we investigated both brain infection and inflammation in a mouse model of VL. Using
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P1644-100ML | 04061838262035 |
| P1644-1L | 04061838262042 |
| P1644-25ML | 04061838262059 |
| P1644-500ML | 04061838262066 |