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Merck
CN

P2069

苯酚:氯仿:异戊醇 25:24:1,10 mM Tris(pH 8.0)、1 mM EDTA饱和

Supplied with Equilibration buffer, Molecular Biology

别名:

DNA extraction

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关于此项目

NACRES:
NA.52
UNSPSC Code:
12190000
Assay:
≥99.0%
Grade:
Molecular Biology
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grade

Molecular Biology

Quality Level

assay

≥99.0%

form

liquid

pH

6.5-6.9(pH of phenolic phase), 7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)

application(s)

agriculture

storage temp.

2-8°C

Application

对于需要更高 pH 值的应用,例如大的完整基因组 DNA的分离,建议添加平衡缓冲液。
苯酚:氯仿:异戊醇已用于制备 RNA,用于研究从心肌活检中分离总 RNA。它还被用于在人雄激素受体(HUMARA) 克隆性检测中提取 DNA。
这批苯酚:氯仿:异戊醇 25:24:1 采用从大肠杆菌中提取 的pBR322 DNA 的粗制品进行了测试。 根据琼脂糖凝胶电泳评估,提取的 DNA 没有降解或浓度降低。 采用相同的操作程序处理纯化的 pBR322 DNA 和 λ Hind III 消化 DNA。 根据琼脂糖凝胶电泳评估,纯化的制剂没有检测到降解或浓度降低。

Packaging

随配 P3803 和一瓶单独的平衡缓冲液,用于将苯酚相调节至 pH 8.0 ± 0.2。

Preparation Note

将小瓶平衡缓冲液的全部内容物添加到大瓶苯酚:氯仿:异戊醇中。 在使用前轻柔地混合并使相实现分离,大约 2-4 小时。 这会将苯酚溶液的 pH 值从 6.7± 0.2 调整到 8.0± 0.2。

Other Notes

添加平衡缓冲液后,产品可稳定 6 个月,而不添加缓冲液可稳定 2 年。 在添加平衡缓冲液后,在 -20 °C 下避光储存可将保质期延长至 1 年以上。


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Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

target_organs

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

易制毒化学品(2类)
危险化学品

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.


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全球贸易项目编号

货号GTIN
P2069-100ML04061837898709
P2069-400ML04061837898723