产品名称
磷酸盐缓冲剂，含0.05% TWEEN® 20，pH 7.4,
form
powder
Quality Level
color
white
solubility
water: soluble, clear, colorless (1 pouch dissolved in 1L)
application(s)
cell analysis
diagnostic assay manufacturing
General description
磷酸盐缓冲液-Tween®20(PBS-T)主要用作免疫分析中的洗涤和封闭缓冲液。磷酸缓冲液用于维持稳定的pH 7.4，适合许多生物反应和试验。PBS-T中的盐或生理盐溶液用于提供维持最佳蛋白溶解度和稳定性需要的离子强度。0.05% TWEEN20是一种非离子去污剂，用于通过封闭蛋白的非特异性结合位点降低背景信号。PBS-T缓冲液通过清除不必要物质提高洗涤效率，并在保持靶蛋白完整性的前提下减少非特异性抗体结合位点。
Application
磷酸盐缓冲液用于：
- 蛋白质免疫印迹分析
- 酶联免疫吸附测定(ELISA)
- 端粒FISH (Tel-FISH)和端粒/免疫染色-FISH (Teli-Fish)
- 细胞分离
Packaging
锡箔袋包装
Preparation Note
一个袋中的内容物溶解于1L蒸馏水或超纯水水中，将得到0.01 M磷酸盐缓冲液、0.138M NaCl、0.0027M KCl、0.05% Tween 20，pH7.4.
Legal Information
TWEEN is a registered trademark of Croda International PLC
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
实验方案
ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P3563-5X10PAK | 04061835547548 |
| P3563-10PAK | 04061835547531 |
| P3563-1EA | 04061824081541 |