grade
Molecular Biology
Quality Level
assay
≥99.0%
form
liquid
pH
6.5-6.9(pH of phenolic phase)
application(s)
agriculture
storage temp.
2-8°C
Application
使用苯酚:氯仿:异戊醇溶液提取，可以从核酸中去除蛋白质。对于核酸粗提混合物，在使用苯酚:氯仿:异戊醇溶液提取前，最好先用一系列蛋白水解酶消解。尽管蛋白质能够被苯酚有效变性，核酸酶活性并未被完全抑制。因此需加入少量异戊醇，进一步确保抑制核酸酶活性。请查阅我们的产品信息表，获得从核酸中去除蛋白质的实验方案。
Other Notes
该产品的保质期是两年。
进行DNA提取时，可以用Tris缓冲液平衡，将苯酚相的pH调整到8.0。Sigma提供专门用于DNA提取的P2069产品。该产品包括了平衡缓冲液，能够方便地调节pH值。
Disclaimer
如果苯酚接触到皮肤，会很快烧伤。因此，使用者需要穿着合适的实验服，包括手套和眼睛护具。如果直接接触到皮肤，立刻用大量水冲洗，并且寻求医学救助。
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signalword
Danger
Hazard Classifications
Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
target_organs
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
危险化学品
易制毒化学品（2类）
此项目有
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Short-read high-throughput DNA sequencing technologies provide new tools to answer biological questions. However, high cost and low throughput limit their widespread use, particularly in organisms with smaller genomes such as S. cerevisiae. Although ChIP-Seq in mammalian cell lines is replacing
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Viral noncoding RNAs have acquired increasing prominence as important regulators of infection and mediators of pathogenesis. Circular RNAs (circRNAs) generated by backsplicing events have been identified in several oncogenic human DNA viruses. Here, we show that Merkel cell polyomavirus (MCV)
Stephan C A Wens et al.
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Elevated plasma cardiac troponin T (cTnT) levels in patients with neuromuscular disorders may erroneously lead to the diagnosis of acute myocardial infarction or myocardial injury. In 122 patients with Pompe disease, the relationship between cTnT, cardiac troponin I, creatine kinase
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P3803-400ML | 04061836853594 |
| P3803-100ML | 04061836853570 |