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Merck
CN

P3910

蛋白酶 来源于地衣芽孢杆菌

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

别名:

Subtilisin A, Subtilisin Carslberg, 枯草杆菌蛋白酶A, 蛋白酶 来源于地衣芽孢杆菌

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-752-2
NACRES:
NA.84
MDL number:
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form

lyophilized powder

Quality Level

quality

for use in Total Dietary Fiber Assay, TDF-100A

mol wt

27 kDa

application(s)

food and beverages

shipped in

wet ice

storage temp.

2-8°C

General description

枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。

Application

这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶,并在碱性条件下具有活性。它用于总膳食纤维检测(TDF-100A)中。

Biochem/physiol Actions

枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白,并在碱性条件下具有活性。
蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活,如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。

Other Notes

在pH 7.5、37°C条件下,一个单位每分钟可水解酪蛋白,生成相当于1.0 μmole (181 μg)酪氨酸的颜色。


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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

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Lisete Paiva et al.
Marine drugs, 16(8) (2018-07-28)
This study evaluates, for the first time, the seasonal (winter and summer) and geographical (São Miguel⁻SMG and Santa Maria⁻SMA Islands) variability of Fucus spiralis (Fs) biochemical composition (dry weight basis) and antioxidant properties. Protein and carbohydrates presented higher values in
Yong Zhuo et al.
Journal of animal science and biotechnology, 11, 47-47 (2020-05-20)
This study aimed to investigate the impacts of guar gum and cellulose as the source of dietary fiber during gestation on the reproductive performance of sows. A total of 210 sows (parities 3-6) were randomly allocated into six diets (n = 35)
H Ikemura et al.
The Journal of biological chemistry, 262(16), 7859-7864 (1987-06-05)
Subtilisin E, an alkaline serine protease of Bacillus subtilis 168, is first produced as a precursor, pre-pro-subtilisin, which consists of a signal peptide for protein secretion (pre-sequence) and a peptide extension of 77 amino acid residues (pro-sequence) between the signal



全球贸易项目编号

货号GTIN
P3910-500MG04061835546312
P3910-5X500MG04061835512898