sterility
aseptically filled
Quality Level
form
colloidal
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
pH
8.5-9.5 (25 °C)
density
1.13 g/mL±0.005 g/mL at 25 °C (lit.)
storage temp.
2-8°C
General description
Percoll®是一种用于细胞、病毒和亚细胞颗粒密度梯度离心的经典培养基。它由直径为15-30 nm（在水中为23％ w/w）并涂覆有聚乙烯吡咯烷酮（PVP）的胶体二氧化硅颗粒组成。PVP涂层使产品完全无毒，非常适合与生物材料一起使用。由于其颗粒大小的不均匀性，沉积以不同的速率发生，自发地在1.0-1.3 g/ml范围内产生非常光滑的等距梯度。
Application
Percoll®已用于以下材料的离心分离：
- 新生大鼠心室心肌细胞
- 血液样本中的白细胞
- 小管周围肌样（PM）细胞
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成，这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用于细胞分离和细胞器分离。
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成，这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用来建立无毒低粘度，低渗透压密度梯度，可用于分离细胞，细胞器和病毒。它已被用来分离 大肠杆菌的内外膜泡。 Percoll在整个梯度过程中都是等渗的，无毒，并且很容易从纯化的材料中去除。
Features and Benefits
- 低渗透压允许对生理条件进行精确调整，而不会受到介质的明显干扰。
- 与活细胞和病毒兼容，并允许分离和恢复完整的，完全活跃的系统。
- 对生物膜不渗透，在离心过程中不改变颗粒的浮力密度。
- 在离心过程中自发形成的梯度允许在离心管中混合大量样品。
- 低粘度导致快速形成梯度和颗粒分离。
- 大多数沉降系数大于60S的生物颗粒可以在Percoll梯度中成功分离。
Legal Information
Percoll is a registered trademark of Cytiva
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
实验方案
为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Cristina Pérez-Patiño et al.
Scientific reports, 9(1), 705-705 (2019-01-27)
The preservation of sperm functional parameters and fertility post-cryopreservation largely varies in the porcine, a species with a fractionated ejaculate. Although intrinsic individual differences have primarily been linked to this variation, differences in protein abundance among frozen-thawed (FT)-spermatozoa are far
Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).
H Pertoft et al.
Analytical biochemistry, 88(1), 271-282 (1978-07-15)
Nunzia Pastore et al.
Nature communications, 11(1), 2461-2461 (2020-05-20)
It is well established that pluripotent stem cells in fetal and postnatal liver (LPCs) can differentiate into both hepatocytes and cholangiocytes. However, the signaling pathways implicated in the differentiation of LPCs are still incompletely understood. Transcription Factor EB (TFEB), a
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P4937-100ML | 04061837036606 |
| P4937-500ML | 04061837036644 |
| P4937-25ML | 04061837036620 |