登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-752-2
MDL number:
eCl@ss:
32160410
Specific activity:
7-15 units/mg solid
Biological source:
Bacillus licheniformis
biological source
Bacillus licheniformis
Quality Level
type
Type VIII
form
lyophilized powder
specific activity
7-15 units/mg solid
mol wt
27 kDa
purified by
crystallization
solubility
10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear, H2O: soluble, colorless
foreign activity
DNase ≤5.0 Kunitz units/mg solid, RNase ≤0.05 Kunitz units/mg solid
storage temp.
−20°C
General description
枯草素 是一种无二硫键的非糖基化单肽链,其分子量为27kda。
该酶在pH 8-10下稳定,可在24小时保存高达90%的活性。其55-60℃下具有最大活性。
Application
该产品已与其他酶一起用于 原位 蛋白水解,以制备适用于结构测定的晶体。它还被用于分离可用于 体外 功能研究的肌膜下(SS)和肌原纤维间(IMF)线粒体。
这是由 衣芽孢杆菌发酵而分离出来的一种蛋白水解酶。这是一种丝氨酸内蛋白酶,对天然和变性蛋白具有广泛的特异性,在碱性条件下具有活性。
Biochem/physiol Actions
枯草素A 是丝氨酸S8内蛋白酶家族的一个成员。它具有广泛的特异性,在P1位置有较大的非荷电残基。它可水解天然和变性蛋白,并且在碱性条件下具有活性。
蛋白酶通过水解肽键分解蛋白质。 蛋白酶可被丝氨酸活性位点抑制剂灭活,如苯基甲基磺酰氟(PMSF)和二异丙基氟磷酸酯。 蛋白酶,P5380的产物,是一种丝氨酸内蛋白酶,对天然和变性蛋白具有广泛的特异性,在碱性条件下具有活性。 它在某些有机溶剂中具有活性,如干辛烷。
Other Notes
一单位在37°C、pH 7.5条件下每分钟可水解1.0μmole(181 μg)的酪氨酸(福林酚试剂显色)。
Still not finding the right product?
Explore all of our products under 蛋白酶 来源于地衣芽孢杆菌
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
此项目有
实验方案
本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像,完成整个免疫组织化学(IHC)操作步骤。
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
L Proctor et al.
Gynecologic oncology, 146(3), 596-602 (2017-06-26)
We sought to determine whether DNA ploidy correlates with the four molecular subgroups of endometrial carcinoma (EC) as determined using ProMisE (Proactive Molecular Risk Classifier for Endometrial Cancer). 90 cases of EC previously characterized by clinicopathological parameters, outcomes, and ProMisE
Christoph Schiklenk et al.
The Journal of cell biology, 217(7), 2383-2401 (2018-05-08)
Although the formation of rod-shaped chromosomes is vital for the correct segregation of eukaryotic genomes during cell divisions, the molecular mechanisms that control the chromosome condensation process have remained largely unknown. Here, we identify the C2H2 zinc-finger transcription factor Zas1
Michael L Mucenski et al.
Scientific reports, 9(1), 4557-4557 (2019-03-16)
The uterus is a remarkable organ that must guard against infections while maintaining the ability to support growth of a fetus without rejection. The Hoxa10 and Hoxa11 genes have previously been shown to play essential roles in uterus development and
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P5380-100MG | 04061835558445 |
| P5380-1G | 04061835506583 |
| P5380-250MG | 04061834379171 |
| P5380-25MG | 04061834379232 |



