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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-603-1
MDL number:
Specific activity:
≥400 units/mg protein (biuret)
Biological source:
bakers yeast
Concentration:
≥3 mg/mL protein Biuret
biological source
bakers yeast
Quality Level
type
Type III
form
ammonium sulfate suspension
specific activity
≥400 units/mg protein (biuret)
mol wt
tetramer 119,500 Da±600
concentration
≥3 mg/mL protein Biuret
technique(s)
activity assay: suitable
color
white
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
yeast ... PGI1(852495)
General description
研究领域:细胞信号传导
磷酸葡萄糖异构酶是一种普遍存在的多功能细胞质蛋白。该二聚体蛋白的分子量约为119,500 ± 600,由四个几乎相同的亚基组成。
磷酸葡萄糖异构酶是一种普遍存在的多功能细胞质蛋白。该二聚体蛋白的分子量约为119,500 ± 600,由四个几乎相同的亚基组成。
Application
来自面包酵母′(酿酒酵母)的磷酸葡萄糖异构酶的用途:
- 在血清甘露糖酶法测定中,用于将果糖-6-磷酸转化为葡萄糖-6-磷酸。
- 作为四酶之一,用于四酶联合反应测定磷酸甘露糖变位酶(PMM)活性 。
- 在葛根果糖含量测定中,将果糖转化为葡萄糖。
Biochem/physiol Actions
磷酸葡萄糖异构酶(PGI)对于D-葡萄糖-6-磷酸和D-果糖-6-磷酸的相互转化至关重要。PGI负责糖酵解的第二步,并参与葡萄糖生成和磷酸戊糖途径。它在细菌和真核生物中高度保守。PGI在烟曲霉(A. fumigatus)存活中起重要作用,在发育、细胞壁葡聚糖生物合成和毒力中发挥多样作用。磷酸葡萄糖异构酶可用作异构酶、神经白细胞素、自分泌运动因子,以及分化和成熟介质。
Physical form
2.6M (NH4)2SO4 中的晶体悬浮液,含 0.1mM 醋酸镁,pH 7.0
Other Notes
一个酶活性单位是指在pH 7.4、温度25 °C条件下,每分钟将1.0 μm的D-果糖-6-磷酸转化为D-葡萄糖-6-磷酸所需的酶量。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Kudzu [Pueraria montana (Lour.) Merr. Variety lobata: A new source of carbohydrate for bioethanol production
Sage RF, et al.
Biomass and Bioenergy, 33, 57-61 (2009)
S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding
J Pozueta-Romero et al.
FEBS letters, 291(2), 233-237 (1991-10-21)
The standardized enzyme coupling method for assaying sucrose synthase activities in the direction of sucrose cleavage was reexamined using enzyme preparations from cultured cells of sycamore (Acer pseudoplatanus L.) and spinach leaves (Spinacea oleracea). Both ATP and Tris, commonly utilized
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P5381-1KU | 04061834379294 |
| P5381-50KU | 04061834379348 |
| P5381-5KU | 04061834379362 |
