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Merck
CN

P5459

蛋白酶 来源于地衣芽孢杆菌

glycerol solution (50%)

别名:

Subtilisin A, Subtilisin Carslberg, 枯草杆菌蛋白酶A, 蛋白酶 来源于地衣芽孢杆菌

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
Specific activity:
≥7 units/mg protein (biuret)
Biological source:
Bacillus licheniformis
Concentration:
≥50 mg/mL
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biological source

Bacillus licheniformis

Quality Level

form

glycerol solution (50%)

specific activity

≥7 units/mg protein (biuret)

concentration

≥50 mg/mL

solubility

0.85% sodium chloride: soluble, clear (Diluted), H2O: miscible

storage temp.

2-8°C

General description

该酶在50℃下、pH 8-10的范围内具有最佳的明胶水解效果。商业上,它在50℃下、pH 6-10之间是稳定的,被用于用于洗衣洗涤剂配方,也用于从照相乳剂中回收银。

Application

使用来自地衣芽孢杆菌的蛋白酶来消化蛾触角叶中的神经外膜;绿棉铃虫
蛋白酶是一种通过水解肽键来分解蛋白的酶。蛋白酶用于降解蛋白质,研究蛋白酶抑制剂和研究热失活动力学。 蛋白酶用于孵育中的核酸分离步骤。来自地衣芽孢杆菌的蛋白酶(商品货号P5459)用于从骨胳肌中分离线粒体时水解肌肉。

Biochem/physiol Actions

蛋白酶通过肽键的水解使蛋白质分解代谢。蛋白酶可被丝氨酸活性位点抑制剂所灭活,如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。

Packaging

包装规格基于蛋白含量。

Other Notes

若无其他说明,则一个酶活性单位是指在pH7.5、37℃条件下每分钟水解酪蛋白并产生相当于1.0 μM (181 μg) 酪氨酸的颜色(使用Folin-Ciocalteu试剂显色)所需的酶量。


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pictograms

Health hazardCorrosion

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

存储类别

10 - Combustible liquids

wgk

WGK 2

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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全球贸易项目编号

货号GTIN
P5459-10G04061832701356
P5459-5G04061834391722