P6534
磷脂酶 A2 来源于猪胰腺
ammonium sulfate suspension, ≥600 units/mg protein
别名:
PLA, 卵磷脂酶 A, 磷脂酰胆碱 2-酰基水解酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-637-7
MDL number:
Specific activity:
≥600 units/mg protein
form
ammonium sulfate suspension
Quality Level
specific activity
≥600 units/mg protein
UniProt accession no.
storage temp.
2-8°C
Gene Information
pig ... PLA2G1B(445525)
General description
磷脂酶 A2 是一种富含二硫键的小分子蛋白，有 124 个残基。 是一种钙依赖性酶。
Application
磷脂酶 A2 已用于磷脂酶试验，并确定大鼠肾近曲小管段 (PTS) 在氧合和缺氧-复氧过程中的活性。
Biochem/physiol Actions
与单体底物相比，它对聚集的底物具有较高的催化活性。
水解 β-两性甘油磷脂的酯键。首选底物为磷脂酰胆碱、磷脂酰乙醇胺及其缩醛磷脂类似物。磷脂酰肌醇和磷脂酰丝氨酸也被水解。其会攻击完整细胞膜上的磷脂。
Physical form
在 3.2 M (NH 4 ) 2 SO 4 溶液 (pH 5.5) 中的混悬液
Analysis Note
双缩脲法测定蛋白质。
Other Notes
在 pH 8.0、37°C 条件下，1 个单位每分钟将 1.0 μmol 大豆 L-α-磷脂酰胆碱水解为 L-α-溶血磷脂酰胆碱和 1 个脂肪酸。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
B van den Berg et al.
The EMBO journal, 14(17), 4123-4131 (1995-09-01)
The lipolytic enzyme phospholipase A2 (PLA2) is involved in the degradation of high-molecular weight phospholipid aggregates in vivo. The enzyme has very high catalytic activities on aggregated substrates compared with monomeric substrates, a phenomenon called interfacial activation. Crystal structures of
Elena Venuti et al.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 16(6), 763-770 (2017-07-26)
Bile salt stimulated lipase (BSSL; Enzyme Commission (EC) number 3.1.1.13) has been a candidate triglyceridase for improving enzyme therapy for pancreatic insufficiency; however, its efficacy is near absent. We hypothesise that similarly to pancreatic lipase, BSSL is inhibited by phospholipids
B van den Berg et al.
Journal of biomolecular NMR, 5(2), 110-121 (1995-02-01)
The three-dimensional structure of porcine pancreatic PLA2 (PLA2), present in a 40 kDa ternary complex with micelles and a competitive inhibitor, has been determined using multidimensional heteronuclear NMR spectroscopy. The structure of the protein (124 residues) is based on 1854
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P6534-10MG | 04061833273708 |
| P6534-1MG | 04061826683811 |
| P6534-5MG | 04061833273715 |