grade
Hybri-Max™
sterility
0.2 μm filtered
product line
BioReagent
form
solution
mol wt
average mol wt 1,450
concentration
50 % (w/v)
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
SMILES string
C(CO)O
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
InChI key
LYCAIKOWRPUZTN-UHFFFAOYSA-N
General description
聚乙二醇（PEG）是环氧乙烷和水通过缩合反应产生的聚合物。
Application
聚乙二醇溶液已用于以下细胞的杂交：
- 辐照骆驼成纤维细胞与A23仓鼠细胞
- 小鼠脾细胞与Sp2/0-Ag 14小鼠骨髓瘤细胞
- 兔脾细胞和细胞系240E-1
推荐用于要求50%聚乙二醇的正常融合方案。
PEG在单克隆抗体生产中用作杂交瘤制备的细胞融合剂，可诱导细胞杂交。
PEG在单克隆抗体生产中用作杂交瘤制备的细胞融合剂，可诱导细胞杂交。
Biochem/physiol Actions
聚乙二醇（PEG）被用于洗涤剂、乳化剂、香皂、金属加工过程、药物制剂和化妆品中。PEG常被用作润滑剂。它也被用于色谱的固定相中。PEG可防止乳液中的晶体形成。
Packaging
在氮气氛围下密封在安瓿瓶中。
Preparation Note
即用型溶液。如果需要浓度较低的溶液，可使用不含钙的无菌DPBS（D5773）稀释。暴露于较低温度后可能会出现一些沉淀物，但随着溶液温度升高，沉淀应该会消失。 如有需要，可以冷冻溶液，但应该先分装再冷冻，以避免反复冻融。
Other Notes
含50％（w/v）聚乙二醇（平均分子量1450）的DPBS溶液，不含钙。
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
212.0 °F - closed cup
flash_point_c
100 °C - closed cup
ppe
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P7181-5X5ML | 04061834394396 |