P8287
邻苯二胺 二盐酸盐
chromogenic, tablet
别名:
1,2-Phenylenediamine tablet, OPD, OPD Tablet
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关于此项目
线性分子式:
C6H4(NH2)2·2HCl
化学文摘社编号:
分子量:
181.06
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
3912045
产品名称
邻苯二胺 二盐酸盐, tablet, 10 mg substrate per tablet
Quality Level
form
tablet
mp
258 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
General description
邻苯二胺(o-PD)是一种无色的非荧光分子。
邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。
Application
在酶联免疫吸附试验(ELISA)中，邻苯二胺盐酸盐用作辣根过氧化物酶偶联二抗的底物。
Preparation Note
将一片药片溶于 pH 5.0 的 0.05M 磷酸盐-柠檬酸盐缓冲液中至所需浓度（通常使用0.4mg/ml 的 OPD 浓度）。使用前，在每 100ml 底物缓冲溶液中加入 40 μl 新鲜的 30％ 过氧化氢，现配现用。片剂分别包装在箔包内。
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signalword
Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
James D Allen et al.
Frontiers in immunology, 12, 707339-707339 (2021-09-04)
Each person has a unique immune history to past influenza virus infections. Exposure to influenza viruses early in life establishes memory B cell populations that influence future immune responses to influenza vaccination. Current influenza vaccines elicit antibodies that are typically
Sara Buezo Montero et al.
Frontiers in cellular and infection microbiology, 10, 377-377 (2020-08-28)
Mosquitoes of the Aedes genus transmit arboviruses of great importance to human health as dengue, chikungunya, Zika and yellow fever. The tiger mosquito Aedes albopictus can play an important role as arboviral vector, especially when Aedes aegypti is absent or
Henry Surendra et al.
Malaria journal, 18(1), 227-227 (2019-07-10)
Analysis of anti-malarial antibody responses has the potential to improve characterization of the variation in exposure to infection in low transmission settings, where conventional measures, such as entomological estimates and parasitaemia point prevalence become less sensitive and expensive to measure.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P8287-100TAB | 04061834743040 |
| P8287-50TAB | 04061834743057 |