P8787
邻苯二胺 二盐酸盐
chromogenic, tablet
别名:
1,2-Phenylenediamine tablet, OPD, OPD Tablet
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关于此项目
线性分子式:
C6H4(NH2)2·2HCl
化学文摘社编号:
分子量:
181.06
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
3912045
产品名称
邻苯二胺 二盐酸盐, tablet, 4 mg substrate per tablet
Quality Level
form
tablet
mp
258 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
General description
邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。
Application
在酶联免疫吸附试验（ELISA）中，邻苯二胺盐酸盐用作辣根过氧化物酶偶联二抗的底物。
Preparation Note
将 1 片片剂溶于 pH 值为 5.0的0.05M磷酸盐 - 柠檬酸盐缓冲液中至所需浓度（通常使用0.4mg / ml的OPD浓度）。临用前，向 100 mL 底物缓冲液中加入 40μl 新鲜过氧化氢（浓度为 30%）。片剂单独包装在铝箔包装中。
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wgk
WGK 3
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1
存储类别
11 - Combustible Solids
商品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P8787-50TAB | 04061834401827 |
| P8787-100TAB | 04061835558858 |