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Merck
CN

P9273

脯氨酸-Arg-7-酰胺基-4-甲基香豆素

≥95%

别名:

PFR-Nmec

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关于此项目

经验公式(希尔记法):
C30H37N7O5 · C2H4O2
化学文摘社编号:
分子量:
635.71
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32
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Quality Level

assay

≥95%

form

powder

composition

Peptide content, ≥65%

solubility

methanol: 50 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

NC(NCCC[C@@H](C(NC1=CC=C(C(C)=CC(O2)=O)C2=C1)=O)NC([C@H](CC3=CC=CC=C3)NC(C4NCCC4)=O)=O)=N.CC([O-])=O

InChI

1S/C30H37N7O5.ClH/c1-18-15-26(38)42-25-17-20(11-12-21(18)25)35-28(40)23(10-6-14-34-30(31)32)36-29(41)24(16-19-7-3-2-4-8-19)37-27(39)22-9-5-13-33-22;/h2-4,7-8,11-12,15,17,22-24,33H,5-6,9-10,13-14,16H2,1H3,(H,35,40)(H,36,41)(H,37,39)(H4,31,32,34);1H

InChI key

QYFPUTDFLKFOBA-UHFFFAOYSA-N

General description

一种胰腺和尿激肽释放酶的荧光底物。

Application

Pro-Phe-Arg-7-氨基-4-甲基香豆素已在以下应用中用作荧光底物:
  • 动力学实验,测定 XII 因子的酶活性
  • 通过Spectramax酶标仪测定制得的血浆激肽释放酶活性
  • 通过Tecan Safire 2酶标仪测定组织蛋白酶 A(CatA)抑制剂对各种蛋白酶的选择性



存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Y Kitamura et al.
Biochimica et biophysica acta, 1387(1-2), 153-164 (1998-09-28)
The culture medium in which prehatching embryos of the frog, Rana pirica, were cultured (hatching medium) solubilized the vitelline coat (VC) of unfertilized eggs and contained molecules reactive to antibodies (anti-UVS.2) against the Xenopus hatching enzyme (HE). The hydrolyzing activity
L Li et al.
Clinical and experimental immunology, 120(2), 261-266 (2000-05-03)
Chemical catalysis, an effector mechanism utilized by fully assembled antibodies, can also be mediated by the isolated antibody subunits. Because trace amounts of free light chains (L chains) are present in IgG preparations, a detailed study was undertaken to identify
H Kato et al.
Journal of biochemistry, 87(4), 1127-1132 (1980-04-01)
Kallikrein in human urine was measured using a fluorogenic peptide substrate, proly-phenyl-alanyl-arginine-4-methylcoumaryl-7-amide (Pro-Phe-Arg-MCA). Using 20 microliters of normal urine, kallikrein activity was quantitatively assayed by incubation with a final concentration of 10(-4) M Pro-Phe-Arg-MCA at 37 degrees C for 90



全球贸易项目编号

货号GTIN
P9273-25MG04061832909585