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P9620

嘌呤霉素

Name: 嘌呤霉素
Brand: SIGMA

Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H₂O, suitable for cell culture

别名:

3′-(L-α-Amino-p-methoxyhydrocinnamamido)-3′-deoxy-N,N-dimethyladenosine dihydrochloride, Stylomycin dihydrochloride

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关于此项目

化学文摘社编号:

58-58-2

NACRES:

NA.85

PubChem Substance ID:

329820352

UNSPSC Code:

51281906

MDL number:

MFCD00012691

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属性

biological source

Streptomyces alboniger

Quality Level

300

type

for cell culture

assay

>98% (HPLC)

form

ready-to-use solution

storage condition

(Store in cool place. Keep container tightly closed in a dry and well -ventilated place. )

concentration

10 mg/mL in H₂O

technique(s)

cell culture | mammalian: suitable

color

colorless to yellow

solubility

H₂O: soluble 10 mg/mL

antibiotic activity spectrum

Gram-positive bacteria, neoplastics, parasites

mode of action

protein synthesis | interferes

shipped in

wet ice

storage temp.

−20°C

SMILES string

Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

InChI key

MKSVFGKWZLUTTO-FZFAUISWSA-N

说明

General description

嘌呤霉素，也称杆菌肽或嘌啉霉素盐酸盐，是一种氨基核酸酶抗生素和蛋白质合成抑制剂。它是由土壤放线菌白黑链霉菌（Streptomyces alboniger）通过一系列酶促反应合成。嘌呤霉素有效抑制原核和真核细胞，其作用原理是干扰RNA功能，从而抑制蛋白质合成。它也充当氨基肽酶和脑啡肽酶的抑制剂。嘌呤霉素可以使细菌、原生动物、水藻和哺乳动物细胞的生长迅速停止，但是含pac基因的细胞有耐药性。 pac基因的基因产物是嘌呤霉素-N-乙酰基转移酶，这种酶通过乙酰化反应使嘌呤霉素失活。

嘌呤霉素与tRNA的含氨基末端结构相似，这一特性令其在蛋白质合成过程中进入核糖体，结合新生多肽链，并使链的延长停止。嘌呤霉素广泛用于细胞培养，可作为以耐嘌呤霉素基因转化的细胞的选择剂。

Application

嘌呤霉素是一种氨基核苷酸抗生素，衍生自白黑链霉菌。它可以选择包含抗性基因嘌呤霉素 N-乙酰基转移酶（PAC）的细胞。它被用在兔模型中，研究处理后的血管平滑肌细胞的生存能力。嘌呤霉素用在体细胞克隆和胚胎移植后产生增强的绿色荧光蛋白（EGFP）转基因仔猪。

Biochem/physiol Actions

作用机制：嘌呤霉素通过提前终止链来抑制蛋白质合成，作为氨基酰基-tRNA 的 3′ 末端的类似物起作用。嘌呤霉素还可作为二肽基肽酶 II（丝氨酸肽酶）和胞质溶胶丙氨酰氨基肽酶的可逆抑制剂起作用。

耐药性机制：嘌呤霉素乙酰转移酶是有效的抗性基因。

抗菌谱：该产品对革兰氏阳性微生物有活性，对耐酸杆菌的活性较低，对革兰氏阴性微生物的活性更低。嘌呤霉素可以防止细菌、原生动物、藻类和哺乳动物细胞的生长，并且起效迅速，可在 2 天内杀死 99％的细胞。

Features and Benefits

适用于多种研究应用的优质抗生素
抗生素和蛋白质合成抑制剂
有效抑制原核和真核细胞
通过干扰RNA功能抑制蛋白质合成
常用作细胞生物学研究中的选择剂

Preparation Note

将该产物以 10 mg/mL 的浓度浓缩在水中。已在转染了 pac 耐药基因的 HeLa 细胞上测试了其生长抑制和细胞选择的能力。

Other Notes

保存于密闭容器内，置于干燥通风处。

如需了解生化试剂系列的更多信息，请填写此表。

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

涉药品监管产品

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实验方案

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

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全球贸易项目编号

货号	GTIN
P9620-10ML	04061838446862