R1383
RPMI-1640 培养基
With ʟ-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture
别名:
Roswell Park Memorial Institute 1640 medium
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产品名称
RPMI-1640 培养基, With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
Application
在各种细胞培养实验中，RPMI-1640 培养基已被用作培养人MCF-7乳腺癌细胞 和人去势抵抗性前列腺癌 (CRPC) 细胞系的细胞培养基。它还被用于培养人胰岛，以评估原代人胰岛中的氧化应激反应。
Preparation Note
补充2.0 g/L碳酸氢钠。
配制成每升培养基中含 8.4 克粉末。
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存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Jiayi Yao et al.
Cancers, 12(8) (2020-08-09)
The acidic pH of the tumor microenvironment plays a critical role in driving cancer development toward a more aggressive phenotype, but the underlying mechanisms are unclear. To this end, phenotypic and genotypic changes induced by adaptation of cancer cells to
Qinxi Ma et al.
mSphere, 4(3) (2019-06-28)
Fatty acids have known antifungal effects and are used in over-the-counter topical treatments. Screening of a collection of gene knockouts in Candida albicans revealed that one strain, carrying a deletion of the transcription factor DAL81, is very susceptible to the
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
全球贸易项目编号
| 货号 | GTIN |
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