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R4130

RPMI-1640 培养基

HEPES Modification, with L-glutamine and 25mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

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关于此项目

UNSPSC Code:
12352200
NACRES:
NA.75
MDL number:
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Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。

Preparation Note

按照每升培养基16.4克粉末配制。
补充2.0 g/L碳酸氢钠。


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存储类别

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

粉末培养基及盐混合物极具吸湿性,应注意防潮。

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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