R4875
核糖核酸酶A 来源于牛胰腺
Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
别名:
胰核糖核酸酶, RNAsea, RNase A, 核糖核酸 3′-嘧啶寡核苷酸水解酶, 核糖核酸酶 I
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关于此项目
化学文摘社编号:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-646-6
MDL number:
Specific activity:
≥50 Kunitz units/mg protein
Biological source:
bovine pancreas
Concentration:
≥60% (RNase A, SDS-PAGE)
biological source
bovine pancreas
Quality Level
type
Type I-A
form
powder
specific activity
≥50 Kunitz units/mg protein
mol wt
~13,700
concentration
≥60% (RNase A, SDS-PAGE)
technique(s)
cell based assay: suitable
impurities
salt, essentially free
suitability
suitable for molecular biology
application(s)
diagnostic assay manufacturing
foreign activity
protease, essentially free
storage temp.
−20°C
SMILES string
[nH]1cncc1CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。
Application
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
Biochem/physiol Actions
核糖核酸酶A是一种内切核糖核酸酶，可在嘧啶核苷酸后切割单链RNA。 它在3'磷酸基末端进行攻击。 核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNA酶还可以水解蛋白质样品中的RNA。 RNase A可被His12和His119的烷基化所抑制并被钾盐和钠盐所活化。
Features and Benefits
我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。
Preparation Note
盐分级和色谱纯化。
Analysis Note
蛋白测定方法：E.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Elizaveta Katorcha et al.
PLoS pathogens, 14(6), e1007093-e1007093 (2018-06-22)
The main risk of emergence of prion diseases in humans is associated with a cross-species transmission of prions of zoonotic origin. Prion transmission between species is regulated by a species barrier. Successful cross-species transmission is often accompanied by strain adaptation
Yang Han et al.
eLife, 7 (2018-08-25)
Epigenetic clocks for mice were generated based on deep-sequencing analysis of the methylome. Here, we demonstrate that site-specific analysis of DNA methylation levels by pyrosequencing at only three CG dinucleotides (CpGs) in the genes Prima1, Hsf4, and Kcns1 facilitates precise
D Joseph-McCarthy et al.
Protein engineering, 9(9), 773-780 (1996-09-01)
One relatively new computational approach to the drug discovery process involves calculating functional group maps of a target structure. Experimental functional group mapping techniques have also recently emerged. In this paper, the structure of RNase A with two bound formates
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R4875-100MG | 04061835558926 |
| R4875-1G | 04061835546350 |
| R4875-500MG | 04061836695446 |
| R4875-5G | 04061836695453 |