R5000
核糖核酸酶A 来源于牛胰腺
Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein
别名:
RNAsea, RNase A, 核糖核酸 3′-嘧啶寡核苷酸水解酶, 核糖核酸酶 I, 胰核糖核酸酶
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关于此项目
化学文摘社编号:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-646-6
MDL number:
Specific activity:
>= 60 Kunitz units/mg protein
Biological source:
bovine pancreas
Concentration:
≥60% (SDS-PAGE)
biological source
bovine pancreas
Quality Level
type
Type II-A
form
solid
specific activity
>= 60 Kunitz units/mg protein
mol wt
~13,700
concentration
≥60% (SDS-PAGE)
technique(s)
cell based assay: suitable
impurities
salt, essentially free
suitability
suitable for molecular biology
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。
核糖核酸酶不水解脱氧核糖核酸，因为脱氧核糖核酸缺乏对环状中间体的形成至关重要的2′-羟基。核糖核酸酶可以水解蛋白质样品中的核糖核酸。胰腺核糖核酸酶A在嘧啶（尿嘧啶或胞嘧啶）磷酸键的 3′-侧。
Application
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
核糖核酸酶A可用于从DNA质粒制备物和蛋白质样品中除去RNA。核糖核酸酶A可用于RNA酶保护测定、去除非特异性结合的RNA、分析RNA序列、水解蛋白质样品中包含的RNA、以及DNA的纯化。来自牛胰腺的核糖核酸酶A已被用于评估人核糖核酸酶-1的杂合酶活性的研究中。 来自牛胰腺的核糖核酸酶A也已被用于研究阳离子交换蛋白置换色谱中基于颗粒的整体柱。
Features and Benefits
我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。
Preparation Note
色谱纯化产品
Analysis Note
蛋白测定方法：E.
通过 SDS-PAGE 测定 RNase A 纯度
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Xia Wang et al.
Nature communications, 8(1), 321-321 (2017-08-24)
Reactivating quiescent cells to proliferate is critical to tissue repair and homoeostasis. Quiescence exit is highly noisy even for genetically identical cells under the same environmental conditions. Deregulation of quiescence exit is associated with many diseases, but cellular mechanisms underlying
Beata Schmidt et al.
Journal of chromatography. A, 1018(2), 155-167 (2003-11-19)
The overall topic of the investigation was the separation of basic proteins by cation exchange displacement chromatography. For this purpose two principal column morphologies were compared for the separation of ribonuclease A and alpha-chymotrypsinogen, two proteins found in the bovine
Elie Saliba et al.
eLife, 7 (2018-03-24)
The yeast Target of Rapamycin Complex 1 (TORC1) plays a central role in controlling growth. How amino acids and other nutrients stimulate its activity via the Rag/Gtr GTPases remains poorly understood. We here report that the signal triggering Rag/Gtr-dependent TORC1
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R5000-1G | 04061836695484 |
| R5000-100MG | 04061836695477 |
| R5000-250MG | 04061836695491 |