R5503
核糖核酸酶A 来源于牛胰腺
Type I-AS, 50-100 Kunitz units/mg protein
别名:
RNAsea, RNase A, 核糖核酸 3′-嘧啶寡核苷酸水解酶, 核糖核酸酶 I, 胰核糖核酸酶
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关于此项目
化学文摘社编号:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-646-6
MDL number:
Specific activity:
50-100 Kunitz units/mg protein
Biological source:
bovine pancreas
biological source
bovine pancreas
Quality Level
type
Type I-AS
form
lyophilized powder
specific activity
50-100 Kunitz units/mg protein
mol wt
~13,700
technique(s)
cell based assay: suitable
impurities
salt, essentially free
suitability
suitable for mRNA or total RNA extracted from cells and tissues
application(s)
diagnostic assay manufacturing
foreign activity
protease, essentially free
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。
Application
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
Biochem/physiol Actions
核糖核酸酶A是一种内切核糖核酸酶，可在嘧啶核苷酸后切割单链RNA。它在3'磷酸基末端进行攻击。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNA酶还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化所抑制并被钾盐和钠盐所活化。
Features and Benefits
我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。
Preparation Note
盐分级和色谱纯化。
Analysis Note
蛋白测定方法：E.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Johanna J Kenyon et al.
Carbohydrate research, 434, 12-17 (2016-10-23)
The structure of the K4 capsular polysaccharide (CPS) from a multiply antibiotic resistant Acinetobacter baumannii isolate D78 was elucidated by chemical and spectroscopical analysis. The K4 repeating unit is composed entirely by aminosugars and the trisaccharide repeating unit of the
P Klappa et al.
European journal of biochemistry, 254(1), 63-69 (1998-07-04)
Using a cross-linking approach, we have demonstrated that radiolabeled model peptides or misfolded proteins specifically interact in vitro with two different luminal proteins in a crude extract from sheep pancreas microsomes. One of the proteins was identified as protein disulphide-isomerase
J J Beintema et al.
Cellular and molecular life sciences : CMLS, 54(8), 825-832 (1998-10-07)
Enzymic properties of members of the ribonuclease A superfamily, like the activity on RNA, the preference for either cytosine or uracil in the primary binding site B1, the preference for the other side of the cleaved phosphodiester bond, the B2
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R5503-10G | 04061836695620 |
| R5503-500MG | 04061836695644 |
| R5503-100MG | 04061835558933 |
| R5503-1G | 04061836695637 |
| R5503-5G | 04061836695651 |