R5886
RPMI-1640 培养基
HEPES modification, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
别名:
Roswell Park Memorial Institute 1640 medium
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产品名称
RPMI-1640 培养基, HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
General description
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
该RPMI-1640培养基补充了HEPES。 HEPES是一种Good′s生理缓冲液，有助于提供比碳酸氢盐缓冲液更稳定的培养基pH。
Application
RPMI-1640培养基已用于：
- 培养人淋巴母细胞系细胞
- 前列腺癌细胞
- 人血单核细胞
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Stephanie Puukila et al.
International journal of radiation biology, 95(8), 1072-1084 (2019-05-30)
Purpose: Diagnostic radiation is an important part of patient care in the Intensive Care Unit; however, there is little data on the acute effects of exposure to these doses. We investigated pulmonary and splenic response 30 minutes, 4 hours or 24 hours after
Marga J Bouma et al.
Stem cell research, 41, 101654-101654 (2019-11-18)
Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4
Katrin Kleinmanns et al.
EBioMedicine, 56, 102783-102783 (2020-05-27)
The completeness of resection is a key prognostic indicator in patients with ovarian cancer, and the application of tumour-targeted fluorescence image-guided surgery (FIGS) has led to improved detection of peritoneal metastases during cytoreductive surgery. CD24 is highly expressed in ovarian
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R5886-24X500ML | 04061836695750 |
| R5886-100ML | 04061836695743 |
| R5886-500ML | 04061836695767 |
| R5886-6X500ML | 04061838259967 |