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Merck
CN

R7388

RPMI-1640 培养基

With HEPES, with ʟ-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

别名:

Roswell Park Memorial Institute 1640 medium

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
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产品名称

RPMI-1640 培养基, Modified, with 20 mM HEPES and L-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

General description

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
RPMI-1640培养基配方基于RPMI-1630系列培养基,利用碳酸氢盐缓冲系统和氨基酸和维生素数量的改变。用于人正常白细胞和肿瘤白细胞的培养。RPMI-1640还支持各种类型的细胞培养物的生长,如72小时植物血凝素(PHA)刺激试验中的新鲜人淋巴细胞。

Application

RPMI-1640培养基已用于培养:
  • 人永生角化细胞、体外转染试验
  • 细胞系和人T细胞母细胞,监控自噬流量
  • 小猪外周血单核细胞(PBMC)



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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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全球贸易项目编号

货号GTIN
R7388-6X500ML04061836696306
R7388-100ML04061836696238
R7388-1L04061836696269
R7388-500ML04061836696276